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KMID : 0861019990140020013
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Korea Journal of Herbology
1999 Volume.14 No. 2 p.13 ~ p.22
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Effect of Radix Glycyrrhizae on Antioxidative activities in Sijunzi-Tang
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김형환/Kim, Hyung-Whan
조수인/이용태/김인락/Cho, Su-In/Lee, Yong-Tae/Kim, In-Rak
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Abstract
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The immune response is dependent on phagocyte function. When this function is deficient or enhanced, the patient is susceptable to a large variety of disease. Enhanced function results in an over active immune response. This study was carried out to investigate whether water extracts of Sijunzi-Tang and Radix Glycyrrhizae have inhibitory effect of neutrophil superoxide generation and cell injury. Superoxide was generated by rabbit polymorphonuclear leukocytes (neutrophils) stimulated by tert-butylhydroperoxide(t-BHP), and was measured by luminol and its detector chemiluminometer. The inhibitory effect of extracts against t-BHP induced superoxide generation was dose-dependent in the range of 0.1~1% concentrations.
Cell injury was estimated by measuring lactate dehydrogenase(LDH), and lipid peroxidation was examined by measuring malondialdehyde(MDA), a product of lipid peroxidation. When rat renal cortical slices were treated I mM t-BHP, water decocted herbs inhibited LDH release from the slices as partially dose depecdent manner. It shows that herbs can reduce cytotoxicity, but over dose can be toxic to the slices. And MDA measurement shows the effect of Sijunzi-Tang and Radix Glycyrrhizae inhibiting cytotoxicity from oxidants, partially depend on preventing lipid peroxidation of cells. HPLC data shows the antioxidative effect of Sijunzi-Tang depends on the effect of Radix Glycyrrhizae. So further studies should be followed to make clear the mechanisms of antioxidative effects.
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